Key features and details | |
Cat. No. | MABL-540 |
Name | Anti-BpaB autotransporter mAbs |
Clone No. | AFD- BpaB#4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC, IF |
Species Reactivity | Burkholderia mallei |
Basic Information | |
Specificity | This antibody is specific for Burkholderia mallei autotransporter BpaB. Autotransporter proteins form one of the largest class of virulence factors in Gram-negative organisms and perform important functions in pathogenesis including flocculation, formation of biofilms , complement resistance, host cell adhesion and entry, intracellular motility and replication, cytotoxicity, and lipolytic activity. |
Alternative Name | Burkholderia mallei BpaB autotransporter; BpaB-4; BpaB 4; BpaB- MAb#4; BpaB MAb#4; BpaB autotransporter of B. mallei |
UniProt | |
Immunogen | This antibody was raised by immunising Balb/c mice with purified his-tagged BpaB . |
Application Notes | Given the function of autotransporters, such as BpaB in pathogenesis and their overall structure, they are excellent targets for developing medical countermeasures (MCM) against pathogenic organisms. A significant portion of AT (passenger domain) is readily accessible for recognition by the immune system as it is exposed on the bacterial surface. Consequently, BpaB#4 antibody is recommended as a potential research target in treatment and biosafety measures associated with B. mallei. Efficient and specific binding of this antibody to BpaB was confirmed via Western blot; BpaB#4 (BpaB-MAb#4) identified this autotransporter in the material from whole cell lysates (WCL), total membrane proteins (TMP) and sarkosyl-insoluble fraction containing OM proteins (OMP) coming from recombinant E.coli with B. mallei BpaB (Zimmerman et al., 2015). |
Antibody First Published | Zimmerman et al. The Autotransporter BpaB Contributes to the Virulence of Burkholderia mallei in an Aerosol Model of Infection. PLoS One. 2015 May 20;10(5):e0126437. doi: 10.1371/journal.pone.0126437. eCollection 2015. PMID:25993100 |
Note on publication | This article describes the generation and characterisation of BpaB#4 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
