Key features and details  | |
Cat. No.  | MABL-531  | 
Name  | Anti-BNYVV P25 mAbs  | 
Clone No.  | AFD- scFvP25  | 
From  | Recombinant Antibody  | 
Isotype  | Engineer antibody  | 
Application  | dotblot, ELISA  | 
Species Reactivity  | BNYVV  | 
Basic Information  | |
Specificity  | Specifically binds to beet necrotic yellow vein virus 25K protein but has some reactivity to sap from plants not infected with BNYVV.  | 
Alternative Name  | P25; 25kDa Protein; Protein P25; 25 kDa non-structural protein; BNYVV 25kDa protein  | 
UniProt  | Q65669  | 
Immunogen  | This antibody was raised by injecting purified P25 into mice.  | 
Application Notes  | This antibody is specific for the BNYVV 25kDa protein. Its binding spcificity has been confirmed in dot blots and western blots (Fecker et al, 1996)  | 
Antibody First Published  | Fecker et al Expression of single-chain antibody fragments (scFv) specific for beet necrotic yellow vein virus coat protein or 25 kDa protein in Escherichia coli and Nicotiana benthamiana Plant Molecular Biology 32: 979-986, 1996. PMID:8980548  | 
Note on publication  | Discusses the scFv fragment to test the efficacy of PelB and PHA tags in higher plants on scFv fragments.  | 
COA Information (For reference only, actual COA shall prevail)  | |
Size  | 100 μg Purified antibody.  | 
Concentration  | 1 mg/ml.  | 
Purification  | Protein A affinity purified  | 
Buffer  | PBS with 0.02% Proclin 300.  | 
Concentration  | 1 mg/ml.  | 
Storage Recommendation  | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.  | 

  