Key features and details | |
Cat. No. | MABL-503 |
Name | Anti-beta 1 integrin mAbs |
Clone No. | AFD- AIIB2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, in vivo, inhibition, IP, FC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | The antibody specifically binds β1 integrin extracellular domain. |
Alternative Name | integrin beta 1; Integrin beta-1-binding protein 1; Integrin cytoplasmic domain-associated protein 1 (ICAP-1) |
UniProt | O14713 |
Immunogen | The original antibody was isolated by immunizing a Lewis rat with JAR choriocarcinoma cells. |
Application Notes | The antibody immunoprecipitated the integrin subunits (Hall et al.,1990). This antibody was used for detection of β1 integrin expressed on CHO cells by flow cytometry (Takada et al., 1993; PMID: 7688727). The original format of the antibody or its F(ab′)2 fragments applied to single cells were capable of down-modulating β1 integrin signaling pathways (Wang et al., 1998; PMID: 9843973 and Weaver et al., 1997; PMID: 9105051). The antibody was used in immunofluorescence characterization of integrins in the HMT-3522 cells (Weaver et al., 1997; PMID: 9105051). The antibody inhibited tumor cell growth in several breast cancer cell lines (T4-2, MDA-MB-231, BT474, SKBR3, and MCF-7) and one nonmalignant cell line (S-1). Further, β1 integrin inhibition resulted in a significant loss of cancer cells, associated with a decrease in proliferation and increase in apoptosis, and a global change in the composition of residual colonies (Park et al., 2010; PMID: 16452209). The antibody was found to reduce the frequency of solid tumor stem cells. The antibody inhibited the growth of colon tumors and reduced the percentage of CD44+ cells. Tumors treated in mice showed a decrease when treated with the antibody.The humanized version of the antibody was constructed, showing potential for therapeutic uses (WO2009009114A3). |
Antibody First Published | Hall et al. The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin. J Cell Biol (1990) 110 (6): 2175–2184. |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
