Key features and details | |
Cat. No. | MABL-499 |
Name | Anti-BCMA mAbs |
Clone No. | AFD- I09 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds human and cynomolgus monkey BCMA. It acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. BCMA expression is highest on terminally differentiated B cells. BCMA is involved in mediating the survival of plasma cells for maintaining long-term humoral immunity. It also activates NF-kappa-B and JNK. The expression of BCMA has been linked to a number of cancers, autoimmune disorders, and infectious diseases. |
Alternative Name | CD269; BCM; TNFRSF17; Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; 15B2; IO9 |
UniProt | Q02223 |
Immunogen | The original antibody 15B2 was generated by immunizing six week old female Ablexis transgenic mice with both rHu BCMA-Fc and cynomolgus BCMA-Fc or adherent 293 cells (Ad293) expressing either Hu BCMA or Cyno BCMA. The original mouse antibody 15B2 was mutated to a germline form using primers designed to mutate four non-germline residues in 15B2, which resulted in the generation of this lead optimized antibody. |
Application Notes | The binding affinity of this antibody to monomeric soluble human BCMA was determined using ProteOn™ XPR36 instrument. It was reported that the original human IgG1 version of the antibody binds soluble human BCMA with an affinity of kd= 9.82nM. The binding of this antibody to membrane bound human BCMA was evaluated using flow cytometry in multiple myeloma and plasma cell leukemia cell lines that endogenously express BCMA (NCI-H929, MM.1S cell lines and Ad293 expressing human BCMA). This antibody was also used in the generation of a antibody-drug conjugate (ADC) comprising a BCMA monoclonal antibody like I09 conjugated to a cytotoxin to check its anti-tumor effects in the treatment of multiple myeloma (US10988546). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
