Key features and details | |
Cat. No. | MABL-487 |
Name | Anti-Bax (active monomer) mAbs |
Clone No. | AFD- 6A7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | In the presence of nonionic detergent, the antibody avidly binds the monomeric form of Bax, but not Bax complexed with either Bcl-XL or Bcl-2. [6A7 ] Bax antibody only recognizes conformationally changed active Bax. |
Alternative Name | Bcl-2-like protein 4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L- 4 |
UniProt | Q07812 |
Immunogen | KLH conjugated to peptides corresponding to amino acids 3–16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3–16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12–24 shared by Bax from both species (CGPTSSEQIMKTGA). |
Application Notes | |
Antibody First Published | Hsu YT, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997 May 23;272(21)：13829-34. PMID：9153240 |
Note on publication | Describes the making of the species-independent Bax monoclonal antibodies, shows it recognised monomeric form of Bax, and analysed by immunoprecipitation followed by immunoblotting. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
