Key features and details | |
Cat. No. | MABL-470 |
Name | Anti-B-catenin mAbs |
Clone No. | AFD- 3B1D11A8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human beta-catenin. The human β-catenin acts as a multi-functional protein that plays a very important role in cell adhesion and Wnt signaling pathway mediated gene expression regulation. Β-catenin on the cell membrane can be combined with the cytoplasmic domain of E- Cadherin (E-CAD) to form an e-cadherin / β-catenin complex, which mediates mutual adhesion between the same type. It acts as a key regulatory molecule in the Wnt signaling pathway and plays an important role in the occurrence / evolution of the embryonic development and tumor. |
Alternative Name | CTNNB1; CTNNB; Catenin beta-1; Beta-catenin; CAT-5H10; CGMCC No. 19685 |
UniProt | P35222 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with human beta-catenin- KLH conjugate in Freund's non-complete adjuvant. |
Application Notes | The binding characterization of this antibody was done using ELISA. The original mouse IgG1 antibody binds human B-catenin with an affinity constant of 4.96 X 10^(9) M^(-1). This antibody was also used in the immunohistochemical analysis of colorectal carcinoma and pancreatic cancer tissues. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody CAT-5H10 (CN112457400). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
