Anti-Acetylaminofluorene mAbs

Cat. No.

MABL-343

Name

Anti-Acetylaminofluorene mAbs

Clone No.

AFD- K16-16

From

Recombinant Antibody

Isotype

Engineer antibody

Application

colony hybridization, dot blot hydridization, PCR, plaque hybridization, Southern blot

Species Reactivity

n/a

Basic Information

Specificity

K16-16 binds to AAF-labelled DNA.  AAF is used to label DNA and RNA by binding covalently at the C8 position of   guanosines; AAF can then be detected using anti-AAF antibodies.

Alternative Name

2-AAF; AAF

UniProt

Immunogen

K16-16 mAb was generated by immnizing  BALB/c mice with acetyl aminofluorene-substituted guanosine (Le Guernet al, 1984). RNA derived from this hybridoma clone was then used to construct a   phage display library and was selected for by its affinity for AAF-labelled   sonicated salmon sperm DNA in an ELISA screen.

Application Notes

K16-16 was used to construct a   recombinant conjugate by fusing PhoAv bacterial phosphatase. The conjugate   was used for one-step immunodetection of DNAs and RNAs in dot-blot, colony   and plaque hybridization and in Southern/northern blot hybridization. It has   also been used to detect PCR products

Antibody First Published

Muller et al. Recombinant single-chain Fv antibody fragment-alkaline phosphatase conjugate for one-step   immunodetection in molecular hybridization. J Immunol Methods. 1999 Jul   30;227(1-2):177-85. PMID:10485265

Note on publication

Describes the construction of a   phage display library and the conjugation of the antiAAF scFv to PhoAv by  recloning the scFv gene into the fusion vector pLIP6v and by producing the   resulting scFv–PhoAv hybrid protein in E. coli. This was then shown to be   used as a one-step method for immunodetection.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.