Anti-A2a mAbs

Cat. No.

MABL-333

Name

Anti-A2a mAbs

Clone No.

AFD-Fab2838

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, crysatallization, in vitro, ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for the intracellular surface of A(2A)AR.

Alternative Name

ADORA2; ADORA2A; Adenosine receptor A2a

UniProt

P29274

Immunogen

The original antibody was raised by immunizing a mouse with purified A2AAR-antagonist ZM241385 complex 3.

Application Notes

The original antibody was used for an ELISA, where it showed it was able to bind its antigen. The antibody was used for a ligand binding assay. Here it showed that the antibody was able to   stop the binding of agonist NECA but not that of antagonist ZM241385. This   suggests that this antibody induces a inactive confirmation of A2AAR. The   structure of the original antibody was determined using x-ray crystallography   (Hino et al., 2012; PMID:22286059). The heavy chain of this antibody was   cloned into a plasmid and HEK293T were transfected. In vitro is showed it was   able to block the A2AR signalling pathway (Zhang et al., 2019;  PMID:32308059). The protein-protein interaction of this antibody with its   antigen was assessed using mCBB CN-PAGE (Suzuki et al., 2018; PMID:29432751).

Antibody First Published

Hino et al. G-protein-coupled   receptor inactivation by an allosteric inverseagonist antibody Nature. 2012 Jan 29;482(7384):237-40. PMID:22286059

Note on publication

The original paper reports the  raising of a mouse monoclonal antibody against human A(2A)AR that prevents   agonist but not antagonist binding to the extracellular ligand-binding   pocket, and describe the structure of A(2A)AR in complex with the antibody Fab   fragment (Fab2838).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.