Key features and details | |
Cat. No. | MABL-329 |
Name | Anti-8-OH-Adenosine mAbs |
Clone No. | AFD- 8A6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IHC |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for 8-OH-adenosine. |
Alternative Name | adenosine; hydroxyadenosine; hydroxy-adenosine;8-hydroxyadenosine; 8 hydroxy adenosine; Ade; 8-Ox-Ade |
UniProt | |
Immunogen | This antibody was raised by immunizing balb/c mice subcutaneously with 8-OH-adenosine conjugated to keyhole limpet hemocyanin. |
Application Notes | 8A6 is recommended for the detection of 8-OH-Adenosine. In an inhibition experiment it was tested whether a ligand for 8-OH-adenosine could inhibit the binding of this antibody to its antigen. It was shown that a concentration betweeen 1 and 5 um ihibits the antibody binding to its antigen (US6900291B2). Further, the ability this antibody to detect 8-OH-Ade lesions in physiological DNA was tested by dot blot (US6900291B2). This antibody was shown to bind to 8-OH-adenosine conjugated to BSA in an ELISA on 96-well Probind plates (US6900291B2). Furthermore, immunohistochemistry was preformed on English sole kidney using this antibody (US2018066071). Fianally, cancerous and benign prostatic cells from PC patients were also probed via immunohistochemistry using this clone (US2018066071). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
