Key features and details | |
Cat. No. | MABL-319 |
Name | Anti-4Ig-B7H3 mAbs |
Clone No. | AFD-8H9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, IP, therapeutic, WB, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody recognizes the 4Ig Domain Isoform of the Human B7-Homolog 3, 4Ig-B7H3. The epitope that the antibody recognizes appears to be restricted to tumors versus normal tissues. |
Alternative Name | 4Ig domain isoform of the human B7-homolog 3; CD276; B7-H3; B7H3; CD276 antigen; B7 homolog 3; 4Ig-B7-H3; costimulatory molecule |
UniProt | Q5ZPR3 |
Immunogen | The antibody was originally generated by immunizing BALB/c mice with human neuroblastoma. |
Application Notes | The antibody was highly reactive with human brain tumors, childhood sarcomas, and neuroblastomas by immunohistochemistry. The antibody was nonreactive with normal human tissues. 4IgB7H3 was immunoprecipitated using this antibody (Modak et al., 2001; PMID: 11358824). In vitro characterization of radiolabeled 8H9 showed that (125)I-8H9 had a K(d) of 10.3nM with an estimated 115,000 binding sites on every HTB82 cell. Further, (125)I-8H9 was retained on the cell surface without significant internalization. In vivo targeting of 125I and 131Ilabeled 8H9 in human RMS xenografts was studied. 125I-8H9 could be used for tumor localization in animals. Instead, mice injected with 131I-8H9 showed a significant suppression in tumor volume (Modak et al., 2005; PMID:16248769). |
Antibody First Published | Modak et al. Monoclonal antibody 8H9 targets a novel cell surface antigen expressed by a wide spectrum of human solid tumors Cancer Res. 2001 May 15;61(10):4048-54. PMID: |
Note on publication | The original paper describes the generation and characterization of the antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
