Key features and details | |
Cat. No. | MABL-308 |
Name | Anti-3-Phenoxybenzoic acid mAbs |
Clone No. | AFD- 3P5ThC12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody is highly specific and sensitive for 3-PBA and its 4-hydroxylated derivative, 4-hydroxy 3-PBA. The antibody does not cross reacts with 3-Phenoxybenzyl aldehyde, 3-PBA-glycine conjugate, 3- Phenoxybenzyl alcohol, Permethrin, Cypermethrin, Esfenvalerate, Deltamethrin, Cyfluthurin. 3-PBA is a human urinary metabolite of pyrethroid insecticides and can be used as a biomarker to monitor human exposure to these pesticides. |
Alternative Name | 3-PBA; 3739-38-6; m-Phenoxybenzoic acid; 3-Carboxydiphenyl ether |
UniProt | |
Immunogen | The original antibody was generated by immunizing an alpaca with 3-PBA, followed by antibody library construction and phage display-based selection. |
Application Notes | The VHH fragment was employed in an indirect competitive VELISA against 3-PBA, showing an IC50 = 1.4 ng/ml. The performance of the VHH fragment was also evaluated using PELISA, the IC50 value for the VHH was 0.6 ng/ml. Both assays showed a similar tolerance to methanol and dimethylsulfoxide up to 50% in assay buffer. The VELISA and PELISA were used for the immunodetection of 3-PBA in human urine samples (Kim et al., 2012; PMID: 22148739). The VHH fragment was used to construct a VHH-alkaline phosphatase (AP) fusion protein. The fusion protein was used to develop a direct competitive fluorescence enzyme immunoassay for detection of 3-PBA in urine. |
Antibody First Published | Kim et al. Isolation of alpaca anti-hapten heavy chain single domain antibodies for development of sensitive immunoassay Anal Chem. 2012 Jan 17;84(2):1165-71 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
