Key features and details | |
Cat. No. | MABL-3119 |
Name | Anti-TNFalpha mAbs |
Clone No. | AFD- Di62 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralising, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human TNFα. |
Alternative Name | TNFSF2; tumor necrosis factor alpha; TNF; C1K3N5; TNFA; Cachectin; TNF-alpha; TNF-a; Tumor necrosis factor ligand superfamily member 2; TNF-α |
UniProt | P01375 |
Immunogen | This antibody was raised against human TNFα. |
Application Notes | This antibody binds to human TNFα with a high affinity (Kd = 0.4 nM) in ELISA analysis, and can block the interaction between TNFα and its receptor (Doring et al, 1994). In addition, this antibody inhibits human TNFα-induced cytotoxicity in several human and mouse cell lines, including murine L929 cells. A peptide derived from the CDR1 of the light chain of this antibody can also inhibit TNFα binding to L929 cells (Doring et al, 1994). |
Antibody First Published | Doring et al. Identification and characterization of a TNF alpha antagonist derived from a monoclonal antibody Mol. Immunol. 31 (14), 1059-1067 (1994) PMID:7935497 |
Note on publication | Describes the original characterisation of this antibody, and its inhibition of the cytokine effect of TNFα |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
