Key features and details | |
Cat. No. | MABL-3117 |
Name | Anti-TNF-alpha mAbs |
Clone No. | AFD-VHH#m3F |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | antagonist, functional assays, ELISA |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for human TNF-alpha but cross-reacts with mouse TNF-alpha. |
Alternative Name | TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF-α |
UniProt | P01375; P06804 |
Immunogen | The original antibody was selected from a llama-derived nanobody phage display library by screening against biotinylated TNF-alpha. |
Application Notes | The original antibody (llama VHH) was screened for affinity to its target via ELISA and identified as antagonistic through functional assays, such as MTT cytotoxicity assays. A bi-valent version of the antibody (termed VHH#3F) was used in an in vivo model for IBD. Treated mice exhibited increased colon length and improved histological scores compared to the negative control (PBS-treated mice), indicating a reduction in inflammation and tissue damage as well as an overall therapeutic effect on chronic colitis symptoms (US9371381B2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
