Key features and details | |
Cat. No. | MABL-3115 |
Name | Anti-TLR7 mAbs |
Clone No. | AFD- rE3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA, FC |
Species Reactivity | Monkey, Human |
Basic Information | |
Specificity | This antibody binds human toll-like receptor 7 (TLR7). It also cross reacts with monkey TLR7. It is an endosomal receptor that plays a key role in innate and adaptive immunity. It also controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. TLR7 is considered to be a useful marker in indicating pathological condition of autoimmune diseases. |
Alternative Name | Toll-like receptor 7; UNQ248/PRO285 |
UniProt | Q9NYK1 |
Immunogen | The original antibody was generated by immunizing wild-type Wister rat with Ba/F3 cell line expressing hTLR7-Flag-Hisx6/hUnc93B1-HAx2 mixed with a suitable adjuvant. |
Application Notes | This antibody was used in combination with another anti-TLR7 antibody called TLM3 to detect human TLR7 in a sandwich ELISA. LTM3 was used as used as a capturing antibody and biotinylated rE3 antibody (rE3-bio) was used a detecting antibody. This antibody can be used for the detection of human TLR7 in serum or plasma derived from human peripheral blood. The binding ability of this antibody for Ba/F3 cell line expressing human TLR7, mouse TLR7 and monkey TLR7 was checked using flow cytometry. This antibody can successfully detect human and monkey TLR7, but not mouse TLR7 in a flow cytometry. This antibody was also used in the detection of soluble human TLR7 in a sample using immunoprecipitation (US20210199667). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
