Key features and details | |
Cat. No. | MABL-3091 |
Name | Anti-Tim-4 mAbs |
Clone No. | AFD- 5G3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, in vivo, IP, ELISA, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine TIM-4. TIM-4, unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen-presenting cells such as lymphoid and myeloid dendritic cells and macrophages. TIM-4 has been shown to interact with TIM-1, thereby suggesting a role of TIM-4 in the balance of Th1/Th2 regulation. Additionally, TIM-4 has been shown to be a receptor for phosphatidylserine and may play a role in the engulfment of apoptotic cells. |
Alternative Name | TIMD4; SMUCKLER; SMUCKLER, TIM4; Spleen, mucin-containing, knockout of lymphotoxin protein; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIM-4; TIMD-4 |
UniProt | Q6U7R4 |
Immunogen | This antibody was generated by immunizing a female Lewis rat with Tim-4-Ig fusion protein. |
Application Notes | The original version of this antibody (rat IgG1) specifically bound murine Tim-4 protein, as shown in ELISA and FC assays. The antibody was also used to immunoprecipitate Tim-4 protein from lysates of CHO-Tim-4 as part of WB assays (Rodriguez-Manzanet et al., 2008; PMID: 18354194). This antibody can block apoptotic body (AB) recognition by CD11b+ peritoneal macrophages and partially inhibit AB uptake by peritoneal B-1 cells (Rodriguez-Manzanet et al., 2010; PMID: 20368430). treatment with this antibody alone did not significantly affect tumor burden or survival, but it enhanced the protective effect of anti-PD-1 blockade. The combined treatment resulted in an increase in total CD8+ and CD8+ CD39 + T cells in the peritoneal cavity. RNA sequencing of sorted Tim-4+ macrophages revealed genes differentially expressed between animals treated with anti-PD-1 alone and those treated with both anti-PD- 1 and this antibody, indicating that it alters the gene expression profile of Tim-4+ macrophages. |
Antibody First Published | Rodriguez-Manzanet et al., TIM-4 expressed on APCs induces T cell expansion and survival J Immunol. 2008 Apr 1;180(7):4706-13. PMID:18354194 |
Note on publication | The original publication characterizes monoclonal antibodies generated to study how TIM-4 induces T-cell expansion and survival. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
