Key features and details | |
Cat. No. | MABL-3082 |
Name | Anti-TIM-2 mAbs |
Clone No. | AFD- RMT2-14 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, functional assays, WB, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine TIM-2, a transmembrane protein that is expressed on activated T cells and preferentially on differentiated Th2 cells. A blockade of TIM-2/TIM-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. TIM-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses. |
Alternative Name | TIMD2; T Cell Immunoglobulin Mucin-2; T Cell immunoglobulin and mucin domain protein 2; TIM3 |
UniProt | Q8R183 |
Immunogen | This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells. |
Application Notes | This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011). |
Antibody First Published | Nakae et al. TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells. Blood. 2007 Oct 1; 110(7): 2565–2568. PMID:17620455 |
Note on publication | Describe the original generation of this clone RMT2-1, together with another clone (RMT2-14), of this anti-mouse TIM-2 antibody and their subsequent use for evaluation of the expression of TIM family members on mouse mast cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
