Anti-TIGIT mAbs

Cat. No.

MABL-3077

Name

Anti-TIGIT mAbs

Clone No.

AFD- MT251

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, FACS, ELISA

Species Reactivity

Human

Basic Information

Specificity

The original antibody is specific for TIGIT.   The antibody does not cross-react with LAG-3, PD-1 and PD-L1.

Alternative Name

VSTM3; VSIG9; WUCAM; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain- containing protein 3

UniProt

Q495A1

Immunogen

The original antibody was generated by   immunizing BALB/c mice with recombinant human TIGIT protein.

Application Notes

The original mouse antibody was subjected to   humanized modification, keeping a similar binding activity. The specificity of the original and the humanized format of the antibody was confirmed by   ELISA analysis (EC50= 0.09901 μg/mL and 0.07412 μg/mL respectively). The   original format of antibody blocked the binding activity of TIGIT and CD155.   The original format and the humanized version of the antibody could recognize   the TIGIT antigen expressed by Jurkat/TIGIT cells by FACS analysis. The   original format of the antibody was able to effectively block the binding of   TIGIT to CD155 (CN112794909).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.