Key features and details | |
Cat. No. | MABL-3070 |
Name | Anti-Thyroid peroxidase mAbs |
Clone No. | AFD- WR1.7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | competition assays, RIA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody specifically targets human TPO, a cell surface enzyme critical for thyroid hormone synthesis. TPO is found in nearly all patients with active autoimmune thyroid disease. |
Alternative Name | TPO |
UniProt | P07202 |
Immunogen | A cDNA library was constructed from the thyroid tissue of patients with Grave's disease, and antibody fragments were expressed in XL1-Blue cells. |
Application Notes | The original form of this antibody (human IgG1) binds human TPO with a Kd of 0.29 nM (Chazenbalk et al., 1993; PMID: 7686925). Competition assays revealed that this antibody binds a distinct epitope compared to clone TR1.9 (Chazenbalk et al., 1993; PMID: 7686925). Further competition studies demonstrated that this antibody inhibits TPO autoantibodies in patients' sera by interacting with serum TPO autoantibodies (Chazenbalk et al., 1993; PMID: 7505290). Alongside clones TR1.9, SP1.4, and TR1.8, this antibody maps to the immunodominant region of TPO recognized by autoantibodies in patient sera (Nishikawa et al., 1994; PMID: 7512572). This antibody has also been employed in flow cytometry to detect cells expressing surface TPO (Jaume et al., 1999; PMID: 10323395). |
Antibody First Published | Chazenbalk et al., 1993. Human organ-specific autoimmune disease. Molecular cloning and expression of an autoantibody gene repertoire for a major autoantigen reveals an antigenic immunodominant region and restricted immunoglobulin gene usage in the target organ. J Clin Invest. 1993 Jul; 92(1): 62–74. PMID:7686925 |
Note on publication | Describes the original production of the antibody and its characterisation through competition assays. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
