Key features and details | |
Cat. No. | MABL-3065 |
Name | Anti-Thy-1 mAbs |
Clone No. | AFD- YKIX 337.217 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Depletion, IP, FC |
Species Reactivity | Dog |
Basic Information | |
Specificity | YKIX 337.217 recognises Thy1 cell surface antigen which is a cell adhesion molecule expressed on thymocytes, peripheral T cells, some B cells, monocytes, and granulocytes. This protein is also expressed on cells in the bone marrow, brain, and kidney, as well as on mesenchymal stem cells. |
Alternative Name | CD90; Thy1 |
UniProt | Q9XT67 |
Immunogen | YKIX 337.217 was prepared by immunizing rats with Con A stimulated canine T cells. |
Application Notes | YKIX 337.217 can be used as a marker for canine T-cells in peripheral blood (note that it has been shown that there is high expression on monocytes, and a weaker expression on granulocytes). The antibody also leads to depletion of circulating T cells (PMID： 8252344). This antibody can also be used for IP studies. |
Antibody First Published | Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens:summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994 Mar;43(3):137-54. PMID:8091414 |
Note on publication | Describes the binding specificity and characterisation (by IF staining and FACS, and IP) of antibodies against canine equivalents of human CD antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
