Key features and details | |
Cat. No. | MABL-3050 |
Name | Anti-TGF beta mAbs |
Clone No. | AFD- 1D11.16.8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, IHC-P, IP, neutralize, WB, IHC |
Species Reactivity | Cow, Dog, Hamster, Monkey, Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody recognizes and binds human, mouse, rat, cow, dog, hamster, monkey and other non-human primate transforming growth factor (TGF) beta 1, 2 and 3. TGF beta is a multifunctional cytokine that exists as five isoforms (TGF beta 1-5) that share 70-80% homology with each other. These proteins are produced by many cell types and function as growth and differentiation factors. |
Alternative Name | TGF-β; TGFβ; TGFB1; TGFB2; TGFB3; TGF-B1; TGF-B2; TGF-B3; TGF beta 1; TGF beta 2; TGF beta 3; CED; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; transforming growth factor; transforming growth factor beta; 1D11.16; 1D11 |
UniProt | P18341; P21214; A6QP91 |
Immunogen | The original antibody was generated by intraperitoneally (IP) injecting female Balb/c mice with 10 ug of native bovine TGF-B2 with complete Freund's adjuvant. |
Application Notes | This antibody was able to neutralize the biological activity of both TGF-beta 1 and beta 2 in vitro. This antibody could detect the dimeric form of TGF-beta 2 in western blots. This antibody was also reported to immunoprecipitate [125I]-TGF-beta 2 (PMID: 2537357). This antibody is also able to neutralize the growth promoting actions of TGF-B1 and B2 in the NRK proliferation assay and TGF-B induced alkaline phosphatase production in an assay using the ROS 17.2.8 osteosarcoma cell line (US5571714). This antibody was used for detection of TGF-β production in vivo in spleens sections of L. donovani-infected hamsters using immunohistochemistry (PMID: 9488418). This antibody was also used for the immunohistochemical analysis of FFPE human renal biopsy specimens for presence of TGF-beta (PMID: 10515378). |
Antibody First Published | Dasch et al. Monoclonal antibodies recognizing transforming growth factor- beta. Bioactivity neutralization and transforming growth factor beta 2 affinity purification. J Immunol. 1989 Mar 1;142(5):1536-41. PMID:2537357 |
Note on publication | Describes the generation and characterization of 4 antibodies against TGF beta. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
