Key features and details | |
Cat. No. | MABL-3034 |
Name | Anti-Testosterone mAbs |
Clone No. | AFD- Nb16 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody is specific for the testosterone molecule. Testosterone is the primary sex hormone and anabolic steroid in males. |
Alternative Name | ChEBI:17347 |
UniProt | |
Immunogen | The antibody was raised by immunizing a young male bactrian camel with testosterone-BSA. |
Application Notes | The binding characterization of this single domain VHH antibody was done using an ELISA on testosterone-BSA. Further a thermofluor experiment was used to determine the Tm value of the nanobody version of this antibody. A surface plasmon resonance was performed to test the binding affinity of the nanobody version of this antibody to testosterone (Li et al, 2016; pmid:27196036). |
Antibody First Published | Guanghui Li et al. Generation of small single domain nanobody binders for sensitive detection of testosterone by electrochemical impedance spectroscopy. ACS Appl Mater Interfaces. 2016 Jun 8;8(22):13830-9. PMID:27196036 |
Note on publication | A phage display library of variable domain of the heavy chain only antibody or nanobody (Nb) was constructed after immunizing a bactrian camel with testosterone. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
