Key features and details | |
Cat. No. | MABL-3031 |
Name | Anti-Testosterone mAbs |
Clone No. | AFD- 77 Fab |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for testosterone, a steroid hormone secreted by the testicles that acts as the principle male sex hormone and anabolic steroid. |
Alternative Name | Fab77; Fab 77 |
UniProt | |
Immunogen | Testosterone. |
Application Notes | This antibody binds to The original version of this antibody (mouse Fab) was shown to bind testosterone with a Kd of 0.3 nM and an ED50 of 4.8 nM (Valjakka et al., 2002; PMID: 12196551). The binding mechanism of this antibody was explored via crystallography (Niemi et al., 2011; 21360611). |
Antibody First Published | Valjakka et al. Crystal structure of an in vitro-affinity- and specificity- matured anti-testosterone Fab in complex with testosterone. Improved affinity results from small structural changes within the variable domains. Journal of Biological Chemistry 2002; 277(46):44021-44027 PMID:12196551 |
Note on publication | The original publication describes the generation and structural analysis of a highly selective, high-affinity anti-testosterone Fab fragment, highlighting small structural changes within the variable domains that result in improved affinity and specificity through comprehensive packing of testosterone with the protein. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
