Anti-SOD1 mAbs

Cat. No.

MABL-2948

Name

Anti-SOD1 mAbs

Clone No.

AFD- D3H5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo, IP, WB, ELISA, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody was specific for misfolded   SOD1 and does not bind to WT SOD1.

Alternative Name

SOD1; SOD1G93A; SOD1 A4V

UniProt

Immunogen

This antibody was raised by immunizing mice   with a recombinant apo form of G93A human SOD1

Application Notes

The original antibody was used for an ELISA on SOD1 WT and misfolded SOD1. It only showed binding to the misfolded SOD1   protein. This antibody was used for a western blot on SOD1 WT and misfolded   SOD1. This antibody was expressed in the spinal cords of mice with a SOD1   mutation simulating ALS. It showed that it delayed disease onset and extended   lifespans (Patel et al., 2014; PMID:24394188). This antibody was used for   immunohistochemistry on spinal cords from transgenic mice over-expressing the   G93A-SOD1 mutation (Gros-Louis et al., 2010; PMID:20345765), and on SOD1G93A   rat spinal cords (Pickles et al., 2016; PMID:27121871). The antibody was used   for immunoprecipitation on spinal cords from transgenic mice over-expressing   the G93A-SOD1 mutation. This antibody was used in vivo on G93A-SO D1 mice. It   delayed the mortality on these mice (Gros-Louis et al., 2010; PMID:20345765).

Antibody First   Published

Gros-Louis et al. Intracerebroventricular   infusion of monoclonal antibody or its derived Fab fragment against misfolded   forms of SOD1 mutant delays mortality in a mouse model of ALS J Neurochem.   2010 Jun;113(5):1188-99. PMID:20345765

Note on publication

In the original paper a passive immunization   approach based on intracerebroventricular infusion in G93A-SOD1 mice of   monoclonal antibodies specific to misfolded forms of SOD1 (mSOD1) was tested.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.