Key features and details | |
Cat. No. | MABL-2946 |
Name | Anti-SOD1 mAbs |
Clone No. | AFD- B12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, WB |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for misfolded SOD1. |
Alternative Name | SOD1; SOD1G93A; SOD1 A4V |
UniProt | |
Immunogen | This antibody was raised from a phage-displayed scFv antibody library |
Application Notes | This antibody was used for a western blot on reacted against denatured wt and mt SOD1. It reacted to denatured wt, A4V and G93A SOD1 proteins but not the control SH3 protein. This antibody was used in vitro on NSC cells to determine the effect of the antibody on A4V and G93A mtSOD1 mediated cell death. The antibody has shown the ability to decrease the cell death caused by A4V and G93A mtSOD1 (Ghadge et al., 2014; PMID:23607939). |
Antibody First Published | Ghadge et al. Single chain variable fragment antibodies block aggregation and toxicity induced by familial ALS-linked mutant forms of SOD1 Neurobiol Dis. 2013 Aug; 56: 74–78. PMID:23607939 |
Note on publication | The original paper generated single chain fragments of variable region antibodies (scFvs) against SOD1, and then expressed them as ‘intrabodies’ within a motor neuron cell line. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
