Key features and details | |
Cat. No. | MABL-2922 |
Name | Anti-SIV-NP mAbs |
Clone No. | AFD- sdAB5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Swine Influenza Virus |
Basic Information | |
Specificity | The antibody is specific for SIV-NP protein. The antibody does not cross-react with PCV2, PEDV, PRRSV, PRV, and CSFV. |
Alternative Name | NP; nucleoprotein; Nucleocapsid protein; Protein N |
UniProt | P16985 |
Immunogen | A VHH library was constructed from peripheral blood mononuclear cells (PBMCs) of an immunized Bactrian camel with SIV-NP recombinant protein, and the antibody was isolated by panning against SIV-NP. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the SIV-NP by western blot analysis. A blocking ELISA based on the biotinylated antibody was developed for SIV detection (Du et al, 2019; PMID: 31819435). |
Antibody First Published | Du et al. Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus Int J Nanomedicine. 2019 Nov 29;14:9337-9349. PMID:31819435 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
