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Anti-sfGFP mAbs

简要描述:

This antibody is specific for superfolder green fluorescent protein which is a better-folding GFP version, with the following mutations: S30R, Y39N, F64L, S65T, F99S, N105T, Y145F, M153T, V163A, I171V and A206V.

产品参数:

Cat. No.:MABL-2894

Species:Engineer

Species Reactivity:​Aequorea victoria (Water jellyfish, Mesonema victoria)

Type:Recombinate

Application:WB, ELISA

详细介绍

Key features and   details


Cat. No.

MABL-2894

Name

Anti-sfGFP mAbs

Clone No.

AFD- NbsfGFP01

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Aequorea victoria (Water jellyfish, Mesonema   victoria)

Basic Information


Specificity

This antibody is specific for superfolder   green fluorescent protein which is a better-folding GFP version, with the   following mutations: S30R, Y39N, F64L, S65T, F99S, N105T, Y145F, M153T,   V163A, I171V and A206V.

Alternative Name

Superfolder green fluorescent protein; GFP;   Green fluorescent protein

UniProt

P42212

Immunogen

The original antibody was generated by immunizing   an adult one-humped camel with recombinant superfolder green fluorescent   protein (sfGFP). An immune nanobody library was generated from the peripheral   blood of the immunized camel. The particular antibody was isolated by panning   the library against sfGFP.

Application Notes

The binding specificity of this nanobody   towards sfGFP was determined using ELISA. This antibody can also be used of  the identification of sfGFP via a western blot (PMID: 25085037).

Antibody First   Published

Twair et al. Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Mol Biol Rep. 2014   Oct;41(10):6887-98. PMID:25085037

Note on publication

Describes the generation of camelid   nanobodies against superfolder GFP.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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