Key features and details | |
Cat. No. | MABL-2894 |
Name | Anti-sfGFP mAbs |
Clone No. | AFD- NbsfGFP01 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Aequorea victoria (Water jellyfish, Mesonema victoria) |
Basic Information | |
Specificity | This antibody is specific for superfolder green fluorescent protein which is a better-folding GFP version, with the following mutations: S30R, Y39N, F64L, S65T, F99S, N105T, Y145F, M153T, V163A, I171V and A206V. |
Alternative Name | Superfolder green fluorescent protein; GFP; Green fluorescent protein |
UniProt | P42212 |
Immunogen | The original antibody was generated by immunizing an adult one-humped camel with recombinant superfolder green fluorescent protein (sfGFP). An immune nanobody library was generated from the peripheral blood of the immunized camel. The particular antibody was isolated by panning the library against sfGFP. |
Application Notes | The binding specificity of this nanobody towards sfGFP was determined using ELISA. This antibody can also be used of the identification of sfGFP via a western blot (PMID: 25085037). |
Antibody First Published | Twair et al. Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Mol Biol Rep. 2014 Oct;41(10):6887-98. PMID:25085037 |
Note on publication | Describes the generation of camelid nanobodies against superfolder GFP. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
