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Anti-Sclerostin mAbs

简要描述:

This antibody binds mouse sclerostin and cross reacts with human sclerostin and recognizes an epitope adjacent to sclerostin‘s binding site for the Wnt co-receptor LRP6. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain which is produced in osteocytes (type of bone cell). The glycoprotein sclerostin has been identified as a negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. Neutralizing anti-sclerostin antibodies are able to restore Wnt activity and enhance bone growth thereby presenting a new osteoanabolic therapy approach for diseases such as osteoporosis.

产品参数:

Cat. No.:MABL-2872

Species:Engineer

Species Reactivity:Human, Mouse

Type:Recombinate

Application:neutralize, ELISA

详细介绍

Key features and   details


Cat. No.

MABL-2872

Name

Anti-Sclerostin mAbs

Clone No.

AFD- AbD09097

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralize, ELISA

Species Reactivity

Human, Mouse

Basic Information


Specificity

This antibody binds mouse sclerostin and   cross reacts with human sclerostin and recognizes an epitope adjacent to   sclerostin's binding site for the Wnt co-receptor LRP6. Sclerostin is a   secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain   which is produced in osteocytes (type of bone cell). The glycoprotein   sclerostin has been identified as a negative regulator of bone growth that   acts through inhibition of Wnt signaling and bone formation. Neutralizing   anti-sclerostin antibodies are able to restore Wnt activity and enhance bone   growth thereby presenting a new osteoanabolic therapy approach for diseases   such as osteoporosis.

Alternative Name

SOST

UniProt

Q9BQB4; Q99P68

Immunogen

The original antibody was isolated from the HuCAL   GOLD library by panning against mouse sclerostin protein biotinylated using   EZ-link Sulfo-NHS-LC-Biotin and coupled to streptavidin-coated magnetic   beads.

Application Notes

The binding reactivity of this antibody for   mouse sclerostin was tested using ELISA. This antibody binds mouse and human   sclerostin with a binding affinity of Kd= 46nM and Kd=260 nM respectively.   This antibody efficiently neutralizes sclerostin's Wnt inhibitory activity.   In vitro interaction analysis using sclerostin variants revealed that this   neutralizing Fab binds to sclerostin's flexible second loop, which has been   shown to harbor the LRP5/6 binding motif (PMID: 27558933).

Antibody First   Published

Boschert et al. The sclerostin-neutralizing   antibody AbD09097 recognizes an epitope adjacent to sclerostin's binding site   for the Wnt co-receptor LRP6. Open Biol. 2016 Aug;6(8):160120. PMID:27558933

Note on publication

This paper describes the generation of   antibodies against human and mouse sclerostin.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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