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Anti-Sag1 mAbs

简要描述:

4F11E12 recognises a highly conserved conformational epitope on the surface of the D1 domain in both native and recombinant SAG1, located within the N-terminal domain; the binding site does not overlap with the proposed ligand-binding pocket or homodimerization site. The eptiope is an immunodominant area and includes residues from 2 long loops located at one exteremity. SAG1 is a GPI- anchored cell surface protein predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. SAG1 recognizes ligands such as glucosamide moieties, which are expressed in a broad range of host cell types.

产品参数:

Cat. No.:MABL-2860

Species:Engineer

Species Reactivity:Toxoplasma gondii

Type:Recombinate

Application:crystallization

详细介绍

Key features and   details


Cat. No.

MABL-2860

Name

Anti-Sag1 mAbs

Clone No.

AFD- 4F11E12

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization

Species Reactivity

Toxoplasma gondii

Basic Information


Specificity

4F11E12 recognises a highly conserved   conformational epitope on the surface of the D1 domain in both native and   recombinant SAG1, located within the N-terminal domain; the binding site does   not overlap with the proposed ligand-binding pocket or homodimerization site.   The eptiope is an immunodominant area and includes residues from 2 long loops   located at one exteremity. SAG1 is a GPI- anchored cell surface protein   predicted to act as an adhesin during host-cell attachment through its   binding to proteoglycans. SAG1 recognizes ligands such as glucosamide   moieties, which are expressed in a broad range of host cell types.

Alternative Name

P30

UniProt

C7E5U4

Immunogen

4F11E12 was generated by intraperitoneal infection of mice with cysts from the 76 K T. gondii strain and was selected for its ability to inhibit efficiently the recognition of SAG1 by sera from infected   patients. The 4F11E12 Fab fragment was created by digestion with papain

Application Notes

F411E12 can be co-crystallized with SAG1,   which enables the mode of binding to be elucidated.

Antibody First   Published

Graille et al. Crystal structure of the   complex between the monomeric form of Toxoplasma gondii surface antigen 1   (SAG1) and a monoclonal antibody that mimics the human immune response. J Mol   Biol. 2005 Nov 25;354(2):447-58. Epub 2005 Sep 30. PMID:16242717

Note on publication

Describes the generation of F411E12 Fab and   its co-crystallization with SAG1. The epitope binding site was determined and   it was found that netiher homodimerization or prevents access to the ligand   binding site (which are necessary to neutralize parasite entry into host   cells).

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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