Anti-RuvC mAbs

Cat. No.

MABL-2856

Name

Anti-RuvC mAbs

Clone No.

AFD- RuvC 5G9/3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB

Species Reactivity

E. coli

Basic Information

Specificity

This antibody recognises E. coli RuvC.

Alternative Name

Crossover junction endodeoxyribonuclease   RuvC; Holliday junction nuclease RuvC; Holliday junction resolvase RuvC; EC   3.1.22.4; 5G9

UniProt

P0A814

Immunogen

This antibody was raised against RuvC   protein from E. coli.

Application Notes

RuvC 5G9/3 (5G9) antibody was initially used   to inhibit RuvC from interacting in a complex with other proteins and   therefore to demonstrate the importance of this complex' formation in the   resolution of Holliday junctions (Eggleston et al., 1997). IP: This antibody was also used to pull down complexes containing RuvC to analyse the   interactions of RuvC with RuvA and RuvB (Davies and West, 1998).

Antibody First   Published

Eggleston et al. In vitro reconstitution of   the late steps of genetic recombination in E. coli. Cell. 1997 May   16;89(4):607-17. PMID:9160752

Note on publication

This article describes experimets for which RuvC 5G9/3 (5G9) antibody was created. The paper reports application of this anti-RuvC antibody to potentially inhibit creation of the RuvABC complex   showing importance of the interactions of RuvC with other proteins in   resolution of Holliday complexes.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.