Key features and details | |
Cat. No. | MABL-2841 |
Name | Anti-RNase A mAbs |
Clone No. | AFD- cAb-RN05 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | calf |
Basic Information | |
Specificity | This antibody binds bovine RNase A. RNase A is an endonuclease enzyme that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. It acts on single-stranded and double-stranded RNA. |
Alternative Name | Ribonuclease pancreatic; RNASE1; RNase 1; RNaseA; RNS1 RN05 |
UniProt | P61823 |
Immunogen | The original antibody was generated by immunizing a dromedary (Camelus dromedarius) with bovine RNase A. The affinity matured version of this antibody was generated using synthetic combinatorial libraries. |
Application Notes | This antibody binds bovine RNase with an affinity in the picomolar range. This affinity matured version of VHH antibody cAb-RN05 binds bovine RNase with an affinity of Kd= 180 pM (PMID: 17888451). 5′–3′ cUMP that binds into the active site of RNase A does not compete with cAb-RN05 for binding to RNase A in an ELISA (PMID: 10196124). |
Antibody First Published | Koide et al. Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope. J Mol Biol. 2007 Nov 2;373(4):941-53. PMID:17888451 |
Note on publication | Describes the affinity maturation of a VVH antibody against bovine RNase. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
