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Anti-RhoG mAbs

简要描述:

The antibody recognizes human, mouse, and rat RhoG. The antibody does not cross react with purified RhoA or Cdc42. RhoG (ARGH) is a member of the Ras super-family of small G-proteins and a member of the Rac subfamily of Rho Family of GTPases. RhoG is a small G-protein (GTPase) who is activated by a GEF (guanine nucleotide exchange factor). The GEF displaces GDP from the binding site of RhoG and allows GTP to bind in its place rendering the protein active. This active RhoG binds downstream effector molecules that include ELMO and DOCK180. RhoG then cleaves GTP to GDP making it inactive.

产品参数:

Cat. No.:MABL-2831

Species:Engineer

Species Reactivity:Rat, Human, Mouse

Type:Recombinate

Application:WB, ELISA, IF

详细介绍

Key features and   details


Cat. No.

MABL-2831

Name

Anti-RhoG mAbs

Clone No.

AFD- 1F3-B3-E5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IF

Species Reactivity

Rat, Human, Mouse

Basic Information


Specificity

The antibody recognizes human, mouse, and   rat RhoG. The antibody does not cross react with purified RhoA or Cdc42. RhoG   (ARGH) is a member of the Ras super-family of small G-proteins and a member   of the Rac subfamily of Rho Family of GTPases. RhoG is a small G-protein   (GTPase) who is activated by a GEF (guanine nucleotide exchange factor). The   GEF displaces GDP from the binding site of RhoG and allows GTP to bind in its   place rendering the protein active. This active RhoG binds downstream   effector molecules that include ELMO and DOCK180. RhoG then cleaves GTP to   GDP making it inactive. 

Alternative Name

Rho-related GTP-binding protein RhoG, Ras   homology Growth-related, ARHG

UniProt

P84095

Immunogen

The antibody was raised by immunizing A/J mice   with amino acids 161-180 of RhoG protein conjugated to KLH.

Application Notes

The specificity of this antibody was   confirmed by ELISA analysis. The antibody recognized RhoG on Western blots   but it did not recognize Rac1 or Cdc42 (Meller et al, 2008; pmid:18505794).   Immunofluorescence was performed on MDA-MB-231 cells using this antibody   (Yamaki et al, 2010; pmid:20679435). Immunofluorescence was also performed on   COS-7 cells (Tzircotis et al, 2011; pmid:21878497).

Antibody First   Published

Meller et al. Endogenous RhoG is dispensable   for integrin-mediated cell spreading but contributes to Rac-independent   migration. J Cell Sci. 2008 Jun 15;121(Pt 12):1981-9. PMID:18505794

Note on publication

 The paper describes the generation and   characterization of the antibody, which is specific for RhoG.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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