Anti-pSYK mAbs

Cat. No.

MABL-2772

Name

Anti-pSYK mAbs

Clone No.

AFD- MIL81-1-8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, ELISA, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for p-Y525-SYK.

Alternative Name

Tyrosine-protein kinase SYK; Spleen tyrosine   kinase; p72-Syk

UniProt

P43405

Immunogen

The original antibody was raised by   immunizing New Zealand White rabbits with a peptide covering residues 520-529   of hSYK and residues corresponding to tyrosine 525 and tyrosine 526 are   phosphorylated.

Application Notes

ELISA was performed on GST-SYK-p using this antibody (WO2015164330). Western blot was performed with this antibody on   GST-pSYK (IVK), and phosphatase- treated GST-pSYK (WO2015164330). AlphaLISA®   luminescence proximity assay was performed on IVK and PPase treated   recombinant GST-SYK samples using this antibody (WO2015164330). This antibody was used for immunofluorescence on HeLa cells transfected for 24 hr with   lipid: DNA complex (WO2015164330).Immunocytochemistry was performed using this antibody on WSU-DLCL2 samples untreated or activated by Fab crosslinking   (WO2015164330). Immunohistochemistry was performed on PHTX-95L   (WO2015164330). This antibody was also used for immunohistochemistry on BLl   xenograft tissue and several primary human tissue xenografts (PHTX-95L, OCI   LY10) (WO2015164330).

Antibody First   Published

Brake et al. Anti-psyk antibody molecules   and use of same for syk-targeted therapy. WO2015164330

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.