Key features and details | |
Cat. No. | MABL-2760 |
Name | Anti-properdin mAbs |
Clone No. | AFD- 3A3E1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | AP-Mediated Hemolytic Assays, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for properdin. It recognizes human, baboon, rabbit, and canine properdin, but not mouse, rat, feline or ferret properdin. |
Alternative Name | factor P; Complement factor P; properdin mab |
UniProt | P27918 |
Immunogen | The original antibody was generated by immunizing mice against purified human properdin. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected only non-reduced properdin as evaluated by western blot analysis. The antibody inhibited AP-mediated hemolysis of rabbit erythrocytes. The ability of the antibody to inhibit the binding of properdin to C3b was tested by flow cytometry. The antibody completely impaired the binding of properdin to C3b-coated erythrocytes (ESC3b) (Chen et al. 2020; PMID: 32793201). Further, the antibody inhibited PGA formation relative to TRAP alone (Blatt et al., 2016; PMID: 27183616). |
Antibody First Published | Chen et al. Properdin Is a Key Player in Lysis of Red Blood Cells and Complement Activation on Endothelial Cells in Hemolytic Anemias Caused by Complement Dysregulation Front Immunol. 2020 Jul 22;11:1460. PMID:32793201 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
