Anti-Profilin-1 & Profilin-2 mAbs

Cat. No.

MABL-2749

Name

Anti-Profilin-1 &   Profilin-2 mAbs

Clone No.

AFD- mAbPRF12a (2-11-H7)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IF, IHC

Species Reactivity

Arabidopsis thaliana

Basic Information

Specificity

This antibody recognizes Profilin-1, Profilin-2   and has a weak binding to Profilin-3. Profilins are actin-binding proteins   involved in the processing of actin cytoskeleton. They are also recognized   allergens.

Alternative Name

Ara t 8; PRO1; Profilin-1; Profilin 1;   Profilin; 2-11-H7; PRF1; 2-11H7; Profilin 2; PRO2; Profilin 3

UniProt

Q42449; Q42418

Immunogen

This antibody was raised by immunising mice   with a purified plant Profilin-1.

Application Notes

mAbPRF12a antibody is recommended for the   analysis and detection of Profilin-1. It strongly identifies Profilin-1 (and   Profilin-2) via Western blot (Kandasamy et al., 2002). This antibody was used   in immunohistochemical and immunofluorescence analyses to identify parts of   the plant where profilins are most prevalent in (Kandasamy et al., 2002).

Antibody First   Published

Kandasamy et al. Plant profilin isovariants   are distinctly regulated in vegetative and reproductive tissues. Cell Motil   Cytoskeleton. 2002 May;52(1):22-32. PMID:11977080

Note on publication

This article describes the generation and   characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.