Key features and details | |
Cat. No. | MABL-2722 |
Name | Anti-porcine VCAM mAbs |
Clone No. | AFD- 3F4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, ELISA, FC |
Species Reactivity | Pig |
Basic Information | |
Specificity | This antibody is specific for an epitope within domain 2 of porcine VCAM, which is expressed on activated endothelial cells. It des not cross-react with human VCAM. |
Alternative Name | pVCAM |
UniProt | F1S567 |
Immunogen | This antibody was raised by immunising BALB/c mice with a recombinant secreted form of porcine VCAM. |
Application Notes | The ability of this antibody to recognise porcine VCAM has been confirmed in ELISA analysis (Mueller et al, 1995). In FC analysis, this antibody reacts with porcine VCAM on TNF-a stimulated porcine aortic endothelial cells, but not to human VCAM on TNF-a stimulated human umbilical vein endothelial cells (Mueller et al, 1995). This antibody blocks VLA-4 dependent adhesion of peripheral blood human T cells to activated porcine aortic endothelial cells (Mueller et al, 1995). It also inhibits the binding of Ramos cells to a recombinant secreted form of porcine VCAM (Mueller et al, 1995). |
Antibody First Published | Mueller et al. Porcine vascular cell adhesion molecule (VCAM) mediates endothelial cell adhesion to human T cells. Developme Transplantation, 1995, Vol.60(11), pp.1299-306 PMID:8525525 |
Note on publication | Describes the original generation of this antibody, and its characterisation in ELISA and FC analysis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
