Key features and details | |
Cat. No. | MABL-2691 |
Name | Anti-Phospholipase A2 mAbs |
Clone No. | AFD- PLA 2-mc8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Trimeresurus stejnegeri (Chinese green tree viper, Viridovipera stejnegeri) |
Basic Information | |
Specificity | This antibody reacts specifically with phospholipase A2 (PLA2) protein present in the venom of Trimeresurus stejnegeri. PLA2 inhibits platelet aggregation induced by ADP, arachidonic acid and PAF. It acts in an enzymatic independent manner on a proteinase-activated receptor (PAR1, F2R) to evoke calcium release through the inositol 1,4,5-trisphosphate receptor (ITPR1, IP3R) and induces mouse aorta contraction. |
Alternative Name | PLA2; Snake venom phospholipase A2; Phospholipase A2 5 |
UniProt | P82896 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with natural phospholipase A2 protein purified from crude venom of Trimeresurus stejnegeri. |
Application Notes | This antibody can be used for the detection of PLA2 protein of Trimeresurus stejnegeri by ELISA. The binding specifcity of the antibody was further confirmed by detection of the protein in a western blot (CN113930408; PMID: 34899734). |
Antibody First Published | Long et al. A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites. Front Immunol. 2021 Nov 9:12:775678. PMID:34899734 |
Note on publication | Describes the generation of monoclonal and polyclonal antibodies against SVMP protein and PLA2 protein found in snake venom. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
