Anti-PD-L2 mAbs

Cat. No.

MABL-2661

Name

Anti-PD-L2 mAbs

Clone No.

AFD- TY25

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, Block, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody binds to mouse PD-L2 and   competes with PD-1 binding. It does not cross-react with PD-L1.

Alternative Name

CD273; B7-DC; PDL2; B7DC; programmed death   ligand 2; CD273; Pdcd1lg2

UniProt

Q9WUL5

Immunogen

This antibody was raised by immunising SD   rats with B7-DC-transfected RAW264.7 cells and selected on B7-DC-transfected   NRK.

Application Notes

This antibody has been used to detect murine   PD-L2 by flow cytometry on stimulated dendritic cells as well as various   mouse tumour cells. It also detects immunoprecipitates mouse PD-L2 and can be   used to detect it by non-reducing Western Blot. TY25 inhibits PD-L2 binding   to PD-1 transfected cells (Ansari et al. 2003, PMID: 12847137) and thereby   blocks PD-L2: PD-1 signalling.

Antibody First   Published

Yamazaki et al. Expression of programmed   death 1 ligands by murine T cells and APC. J Immunol. 2002 Nov   15;169(10):5538-45 PMID:12421930

Note on publication

Describes the generation and initial   characterisation of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.