Anti-PD-L1 mAbs

Cat. No.

MABL-2657

Name

Anti-PD-L1 mAbs

Clone No.

AFD- mAb #18 (PD-L1.A)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo, inhibition, SPR, WB

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human PD-L1.

Alternative Name

mAb #18; CD274; PDL1; B7-H1; B7H1; B7   homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1   ligand 1; Programmed death ligand 1

UniProt

Q9NZQ7

Immunogen

The original antibody was generated by   immunizing BALB/c mice with PD-L1.

Application Notes

The antibody could significantly inhibit the binding of PD-1 and PD-L1 at the cellular level. The humanized version of the antibody was constructed. The humanized version of the antibody detected the   PD-L1 by western blot analysis. The binding affinity of the mouse and   humanized version of the antibody to PD-L1 was measured by surface plasmon   resonance, the affinity was respectively 8.81 nM 7.77 nM. In vivo studies   demonstrated that the humanized version of the antibody could effectively   inhibit tumour growth showing potential tumour therapeutic value   (CN109232740). This antibody was also used in the generation of a bispecific   antibody (BsAb) in a dual-variable-domain immunoglobulin (DVD-Ig) format   targeting CD47 and PD-L1. The variable-domain from the PD-L1-blocking mAb #18   was introduced in tandem onto h4C1 to generate a dual variable domain bsAb   that binds bivalently to both CD47 and PD-L1.

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.