Anti-PD-L1 mAbs

Cat. No.

MABL-2655

Name

Anti-PD-L1 mAbs

Clone No.

AFD- D7A8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, in vivo, SPR, ELISA

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for PD-L1.

Alternative Name

CD274; B7-H1; hPD-L1; PDCD1 ligand 1; Programmed   cell death 1 ligand 1; B7 homolog 1

UniProt

Q9NZQ7

Immunogen

The original antibody was isolated by   panning a phage display library against PD-L1.

Application Notes

The scFv format of the antibody,together   with B1C4A7, was used for constructing in the bispecific antibody   (A7-A8)-LALA. The scFv format and the bispecific antibody could strongly bind   to the recombinant human and mouse PDL1 (EC50 = 0.121 nM and 0.172 nM,   respectively for scFv and 0.088 nM and 0.160 nM, respectively for the   bispecific antibody). The scFv format and the bispecific antibody could   strongly block ligand PDL1 binding to its receptor PD1. IC50 for hPDL1-hPD1   interaction was 2.51 nM for the bispecific antibody, and 3.04 nM for   dsD7A8-LALA. IC50 for the mPDL1-mPD1 interaction was 5.59 nM for the   bispecific antibody, versus 2.80 nM for dsD7A8-LALA. The bispecific antibody   could bind to cell expressed human PDL1 and mouse PDL1. 

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.