Anti-PD-1H mAbs

Cat. No.

MABL-2645

Name

Anti-PD-1H mAbs

Clone No.

AFD- MH5A

From

Recombinant Antibody

Isotype

Engineer antibody

Application

therapeutic, Block, ELISA, IHC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody specifically binds PD-1H.

Alternative Name

VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set  immunoregulatory receptor

UniProt

Q9D659

Immunogen

The antibody was generated by immunising   Armenian hamsters with mPD-1HIg.

Application Notes

The antibody has been labelled with biotin   and used to stain formalin-fixed tissues from wild-type C57BL/6 mice at 6   weeks of age. This showed specific staining for PD-1H in the T cell zone and   marginal zone. The antibody stains P815 cells transfected with full-length   PD-1H plasmid, but not control plasmid. Antibody specificity has been   confirmed by ELISA. A single 200-μg dose of MH5A prevented aGVHD in mouse   models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T   cells in spleens and livers and greatly reduced numbers of inflitrating T   cells in all aGVHD target tissues examined. Few mAbs targeting a single   cosignalling molecule have such a potent preventative effect (Flies D et al, 2011).

Antibody First   Published

Flies D et al. Cutting Edge: A Monoclonal   Antibody Specific for the Programmed Death-1 Homolog Prevents   Graft-versus-Host Disease in Mouse Models J Immunol August 15, 2011, 187 (4)   1537-1541 PMID:21768399

Note on publication

Describes the generation of the antibody and   characterisation of its theraputic function in models of graft-versus-host   disease.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.