Anti-Parvalbumin mAbs

Cat. No.

MABL-2612

Name

Anti-Parvalbumin mAbs

Clone No.

AFD- 3E1G12B6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Fish

Basic Information

Specificity

This antibody is specific for fish   parvalbumin. Its epitope is IKKAFAVID. In muscle, parvalbumin is thought to   be involved in relaxation after contraction. It binds two calcium ions.

Alternative Name

Parvalbumin; PRV7; 3E1

UniProt

E3TEC5

Immunogen

The mouse version of this antibody was raised   by immunizing a mouse with heat-treated catfish sarcoplasmic protein extract.

Application Notes

Both a western blot and an ELISA were   performed on fish proteins using the mouse version of this antibody to   characterize the binding of this antibody (Gajewski and Hsieh, 2009;   pmid:19435232). To study relationships among matrix effect, extractability,   and thermostability during in vitro immunodetection, a western blot was   performed on both crude fish extract and purified parvalbumin (Keshavarz et   al, 2019; pmid:30372974). To analyse the immunochemical properties of pepsin   digested tropomyosin peptide fragments from Atlantic salmon and flathead grey   mullet, a western blot was performed on purified parvalbumin (Keshavarz et   al, 2020).

Antibody First   Published

Gajewski and Hsieh Monoclonal antibody   specific to a major fish allergen: parvalbumin J Food Prot. 2009   Apr;72(4):818-25. PMID:19435232

Note on publication

An antibody was generated against the fish   allergen parvalbumin and further characterized.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.