Key features and details | |
Cat. No. | MABL-2606 |
Name | Anti-paraoxon (organophosphate) mAbs |
Clone No. | AFD-A.17 L47K |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Catalyzation, crystallization |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for paraoxon. |
Alternative Name | L-Leu47Lys; A17 L47K; POX |
UniProt | |
Immunogen | The original antibody was generated via phage display from the Griffin.1 library by panning against reactive phosphonate ester 1. |
Application Notes | This antibody is a covalent trap for Paraoxon and other organophosphate substrates, catalyzing the hydrolysis reaction through the SN2 mechanism. The catalytic parameters of this antibody were measured, and its KD was found to be 170 ± 8 μM; it exhibited a 340-fold reactivity enhancement for paraoxon relative to wild-type Ig-paraoxonase. Furthermore, its crystal structure in complex with paraoxon was determined (Mokrushina et al., 2020; PMID: 32859757). |
Antibody First Published | Mokrushina et al. Multiscale computation delivers organophosphorus reactivity and stereoselectivity to immunoglobulin scavengers Proc Natl Acad Sci U S A. 2020 Sep 15;117(37):22841-22848. doi: 10.1073/pnas.2010317117. Epub 2020 Aug 28 PMID:32859757 |
Note on publication | The original publication explores the enhancement of immunoglobulin (Ig) catalytic activity against organophosphorus toxins through quantum mechanics/molecular mechanics (QM/MM) maturation. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
