Key features and details | |
Cat. No. | MABL-2577 |
Name | Anti-p150glued mAbs |
Clone No. | AFD- S11E |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the first coiled-coil region of the microtubule-associated protein p150glued. |
Alternative Name | p150glued; p150-glued; DAP-150; DP-150; p135; Dynactin subunit 1; 150 kDa dynein-associated polypeptide |
UniProt | |
Immunogen | The original antibody was generated via phage display from the Griffin.1 library by screening against Microtubule-Binding Proteins (MBPs) derived from HeLa S3 cells. |
Application Notes | The original format of this antibody (scFv) was successfully used in a Western blot and for in vitro immunofluorescence staining of p150glued in HeLa cells (Nizak et al., 2003; PMID: 14617357). |
Antibody First Published | Nizak et al. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo Traffic. 2003 Nov;4(11):739-53. doi: 10.1034/j.1600- 0854.2003.00132.x PMID:14617357 |
Note on publication | The original publication demonstrates the use of recombinant antibodies selected through phage display against subcellular fractions. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
