Anti-P-Cadherin mAbs

Cat. No.

MABL-2568

Name

Anti-P-Cadherin mAbs

Clone No.

AFD- 6A9

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for P-Cadherin-1   (CDH3). P-Cadherin-1 is a calcium-dependent cell-cell adhesion glycoprotein   comprised of five extracellular cadherin repeats, a transmembrane region and   a highly conserved cytoplasmic tail.

Alternative Name

Cadherin 3 type 1; Cadherin-3; Cadp; Calcium   dependent adhesion protein placental; CDH3; CDHP; HJMD; P-cadherin   (Placental); PCAD; Placental cadherin

UniProt

P22223

Immunogen

The original antibody was generated by   immunizing mice against a 72-kilodalton (kDa) P- cadherin fragment from A-431 cells.

Application Notes

The antibody detected human P-Cadherin from   A431 cell lysate by Western blot analysis. The antibody was used to   immunoprecipitate P-Cadherin. Further, the antibody was shown to have   inhibitory activity, by disrupting P-cadherin-mediated cell-cell adhesion   with A-431 cells (Lewis et al., 1994; PMID: 8006450). The antibody detected   P-cadherin by immunofluorescence (Owens et al., 2000; PMID: 10708559).

Antibody First   Published

Lewis et al. Cadherin function is required   for human keratinocytes to assemble desmosomes and stratify in response to   calcium J Invest Dermatol. 1994 Jun;102(6):870-7. PMID:8006450

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.