Anti-OX40L mAbs

Cat. No.

MABL-2564

Name

Anti-OX40L mAbs

Clone No.

AFD- OX-89

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, FC, IHC-Fr

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine CD252   (the ligand for OX40), expression of which is restricted to activated B   cells, dendritic cells, microglia and endothelial cells.

Alternative Name

CD252; Tumor necrosis factor ligand   superfamily member 4; OX40 ligand; CD134 ligand; OX40R

UniProt

P43488

Immunogen

This antibody was prepared by immunizing PVG   rats with a recombinant chimeric protein consisting of domains 3 and 4 of rat   CD4 and the extracellular region of mouse CD134L.

Application Notes

This antibody binds mouse CD134L, and has   been shown to bind activated B cells and DCs, as well as CD134L-transfected   fibroblast cells, though not activated T cells, in labelling and   immunohistochemical analysis (Malmström, 2001). In addition, this antibody   is able to partially block the interaction between CD134 and CD134L. This   blocking effect could be responsible for the ability of this antibody to   prevent T cell accumulation in the intestine of T cell-restored SCID mice and   inhibits the development of Th1-mediated colitis.

Antibody First   Published

Malmström et  al. CD134L Expression on Dendritic Cells in the Mesenteric Lymph Nodes Drives   Colitis in T Cell-Restored SCID Mice J Immunol. 2001 Jun 1;166(11):6972-81.   PMID:11359859

Note on publication

Describes the original use of this antibody   in blocking studies to implicate CD134L expression on DCs in mouse mesenteric   lymph nodes in the development of colitis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.