Key features and details | |
Cat. No. | MABL-2512 |
Name | Anti-NP mAbs |
Clone No. | AFD- C6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, in vitro, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for NP but also shows reactivity to (4-hydroxy-3-nitrophenyl)acetic acid. |
Alternative Name | (4-hydroxy-3-nitrophenyl)acetyl; NPA; NO2HPA; 53793; 4-hydroxy-3- nitrophenylacetyl |
UniProt | |
Immunogen | The original antibody was raised by immunizing a C57BL/6 mouse with NP-CGG. |
Application Notes | The original antibody was used for an ELISA on NP-BSA. The antibody was used for affinity measurements using fluorescence quenching on NP-Cap and NP-Gly (Taketani et al., 1995; PMID:7477004). The structure of the original antibody was determined using x-ray crystallography (Nishiguchi et al., 2019; PMID:31521018). |
Antibody First Published | Taketani et al. Role of conserved amino acid residues in the complementarity determining regions on hapten-antibody interaction of anti-(4-hydroxy-3-nitrophenyl) acetyl antibodies Mol Immunol. 1995 Sep;32(13):983-90. PMID:7477004 |
Note on publication | In the original paper monoclonal antibodies (mAbs) specific to (4-hydroxy-3- nitrophenyl)acetyl (NP) were prepared at various times after immunization and the amino acid sequences of VH and V lambda 1 in these mAbs were deduced from cDNA nucleotide sequences. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
