Key features and details | |
Cat. No. | MABL-2510 |
Name | Anti-Notch 4 mAbs |
Clone No. | AFD- GLA-S4F18 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody is capable of binding both human and mouse notch 4. Ths binding epitope of this antibody is located in the negative regulatory region or the NRR. |
Alternative Name | hNotch4; Neurogenic locus notch homolog protein 4 |
UniProt | Q99466 |
Immunogen | The original antibody was isolated from a phage display library generated from a non immunized donor and panning against human and mouse Notch4-negative regulatory region or NRR antigens. |
Application Notes | The original human IgG1, GLA-S4F18 antibody binds human and mouse notch 4 with an EC50 of 0.09 nM and 0.11 nM respectively as measured by direct ELISA. The binding of this antibody to surface expressed notch 4 was determined by flow cytometry. This antibody was reported to significantly delay tumor regrowth when administered in combination with carboplatin in an ovarian xenograft tumor model. When used in combination with cisplatin it delayed tumor regrowth in ovarian PDX models. It also resulted in reduction in cancer stem cells (CSC) present in tumors when treated with GLA-S4F18 alone or in combination with abraxane (WO2018039107). |
Antibody First Published | WO2018039107 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
