Anti-NMDA Receptor mAbs

Cat. No.

MABL-2491

Name

Anti-NMDA Receptor mAbs

Clone No.

AFD-17

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallography

Species Reactivity

Rat

Basic Information

Specificity

Reacts specifically with rat GluN2B ATD.

Alternative Name

NMDAR; NMDA-R; Glutamate receptor

UniProt

Q00960

Immunogen

Monoclonal antibodies that bind rat GluN2B   ATD were obtained by immunizing mice with the purified intact GluN1-GluN2B   NMDA receptors. IgGs were purified from hybridoma cell culture supernatant by   Protein-A Sepharose. Fab fragments have been prepared by papain proteolysis   followed by re- chromatographing onto Protein-A Sepharose to remove Fc.

Application Notes

This antibody has been used for the   crystallisation of the NMDA receptor in the absence of an allosteric   inhibitor at 2.9A (Tajima 2016).

Antibody First   Published

Tajima et al. Activation of NMDA receptors   and the mechanism of inhibition by ifenprodil Nature. 2016 May 2; 534(7605):   63–68 PMID:27135925

Note on publication

Describes the lack of high resolution   structures representing the active state of the NMDA receptor.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.