Key features and details | |
Cat. No. | MABL-2484 |
Name | Anti-NKG2D mAbs |
Clone No. | AFD- CX-5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, blocking, neutralizing |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody recognizes mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1 thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells. |
Alternative Name | CD314; KLRK1; NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; CX5 |
UniProt | O54709 |
Immunogen | The original rat IgG1 version of this antibody was raised by immunizing rats against mouse KLRK1. |
Application Notes | It has been shown that CX5 is a non-depleting antibody, which blocks binding of NKG2D to its ligands and mediates internalization of the receptor. This antibody has been widely used to block NKG2D (Wang et al., 2018; pmid: 29447423; Yang et al., 2017; pmid: 28455530) as well as to detect NKG2D-posotive cells via flow cytometry (Hosomi et al., 2017; pmid: 28747426). |
Antibody First Published | Ogasawara et al. Impairment of NK cell function by NKG2D modulation in NOD mice Immunity. 2003 Jan;18(1):41-51. doi: 10.1016/s1074-7613(02)00505-8. PMID:12530974 |
Note on publication | Describes the characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
