Key features and details | |
Cat. No. | MABL-2477 |
Name | Anti-NK1.1 mAbs |
Clone No. | AFD- PK-136 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, in vivo NK cell depletion, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody reacts with murine NK1.1. NK1.1 is a type II integral membrane glycoprotein with a C-type lectin domain. NK1.1 plays a stimulatory role on natural killer (NK) cells cytotoxicity. Its activation by cross-linking of the receptor induces Ca2+ mobilization and interferon-gamma production. |
Alternative Name | CD161c; Killer cell lectin-like receptor subfamily B member 1C; CD161 antigen-like family member C; Lymphocyte antigen 55c; Ly-55c; NK1.1; NKR-P1.9; NKR-P1C; Natural killer cell surface protein P1-40; NKR-P1 40; PK136 |
UniProt | P27814 |
Immunogen | The original antibody was generated by immunizing (C3H X BALB)F1 mice with spleen cells enriched for NK-1 cells and bone marrow cells from CE mice. |
Application Notes | The antibody was employed for FACS analysis of CD4+8- thymocytes (Arase et al., 1993; PMID: 8419184). The antibody was used for detection of NK1.1 of spleen cells by flow cytometry (Christianson et al., 1996; PMID: 8806787). The antibody was further used for the detection of NK1.1 by flow cytometry analysis (Arase et al., 1992; PMID: 1378629 and Lee et al., 2018; PMID: 30333822 and Coquet et al., 2008; PMID: 18685112). The antibody was employed for in vivo studies, the efficacy of the antibody in depleting the animals of ΝΚ1.1+ or NK T cells was ascertained by flow cytometric analysis. The reduction was greater than 90% after 1 week of treatment (Nomizo et al., 2005; PMID: 16162272). Finally, the antibody was used for the depletion of NK1.1+ cells in euthymic and thymectomized young C57Bl/6 mice infected with T. cruzi. The depletion of NK1.1 cells caused an increase in the parasitemia in young euthymic mice, but produced no such effects in thymectomized young mice infected with T. cruzi (Tulahuen strain) (Cardillo et al., 2002; PMID: 11841697). |
Antibody First Published | Koo et al. Establishment of monoclonal anti-Nk-1.1 antibody Hybridoma . Fall 1984;3(3):301-3. PMID:6500587 |
Note on publication | The original paper describes the generation and characterization of the original antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
